无菌室应根据既经济又科学的原则来设置。其基本要求有以下几点：
Aseptic rooms should be set up according to both economic and scientific principles. The basic requirements are as follows:
1.无菌室应有内、外两间，内间是无菌室，外间是缓冲室。房间容积不宜过大，以便于空气灭菌。内间面积2×2.5＝5m2，外间面积1×2＝2m2，高以2.5m以下为宜，都应有天花板。
1. The aseptic room shall have two rooms, inside and outside. The inside is aseptic room and the outside is buffer room. The room volume should not be too large to facilitate air sterilization. Internal area 2 × 2.5 ＝ 5m2, external area 1 × 2 ＝ 2M2, the height should be less than 2.5m, and there should be a ceiling.
2.内间应当设拉门，以减少空气的波动，门应设在离工作台更远的位置上；外间的门也用拉门，要设在距内间更远的位置上。
2. Sliding door shall be set in the inner room to reduce air fluctuation, and the door shall be set at the farthest position from the workbench; The door of the outer room shall also be sliding door, which shall be set at the farthest position from the inner room.
3.在分隔内间与外间的墙壁或“隔扇”上，应开一个小窗，作接种过程中必要的内外传递物品的通道，以减少人员进出内间的次数，降低污染程度。小窗宽60cm、高40cm、厚30cm，内外都挂对拉的窗扇。
3. A small window shall be opened on the wall or "partition" separating the inner and outer rooms as a necessary channel for internal and external transmission of goods during the vaccination process, so as to reduce the number of personnel entering and leaving the inner room and reduce the degree of pollution. The small window is 60cm wide, 40cm high and 30cm thick, and opposite sashes are hung inside and outside.
4.无菌室容积小而严密，使用一段时间后，室内温度很高，故应设置通气窗。通气窗应设在内室进门处的顶棚上（即离工作台更远的位置），为双层结构，外层为百叶窗，内层可用抽板式窗扇。通气窗可在内室使用后、灭菌前开启，以流通空气。有条件可安装恒温恒湿机。
4. The volume of the sterile room is small and tight. After use for a period of time, the indoor temperature is very high, so a ventilation window should be set. The ventilation window shall be set on the ceiling at the entrance of the inner room (i.e. the farthest position from the workbench), which is a double-layer structure, the outer layer is a shutter, and the inner layer can use a draw plate window sash. The ventilation window can be opened after the inner chamber is used and before sterilization to circulate air. If possible, a constant temperature and humidity machine can be installed.
无菌室工作规程
Working procedures for aseptic room
1.无菌室灭菌。每次使用前开启紫外线灯照射30min以上，或在使用前30min，对内外室用5%石炭酸喷雾。
1. Sterilization in sterile room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5% carbolic acid spray in the interior and exterior chambers.
2.用肥皂洗手后，把所需器材搬入外室；在外室换上已灭菌的工作服、工作帽和工作鞋，戴好口罩，然后用2%煤酚皂液将手浸洗2分钟。
2. After washing hands with soap, move the required equipment into the outer room; Put on sterilized work clothes, hats and shoes in the outer room, wear masks, and then soak your hands with 2% coal phenol soap for 2 minutes.
3.将各种需用物品搬进内室清点、就位，用5%石炭酸在工作台面上方和操作员站位空间喷雾，返回外室，5～10min后再进内室工作。
3. move all necessary articles into the inner room for inventory and position. Spray the 5% carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
4.接种操作前，用70%酒精棉球擦手；进行无菌操作时，动作要轻缓，尽量减少空气波动和地面扬尘。
4. Wipe hands with 70% alcohol cotton ball before inoculation; During aseptic operation, the action shall be gentle to minimize air fluctuation and ground dust.
5.工作中应注意安全。如遇棉塞着火，用手紧握或用湿布包裹熄灭，切勿用嘴吹，以免扩大燃烧；如遇有菌培养物洒落或打碎有菌容器时，应用浸润5%石炭酸的抹布包裹后，并用浸润5%石炭酸的抹布擦拭台面或地面，用酒精棉球擦手后再继续操作。
5. Pay attention to safety during work. If the tampon catches fire, hold it tightly with your hands or wrap it with a wet cloth. Do not blow it with your mouth to avoid expanding the combustion; In case of bacterial culture spilling or breaking the container, wrap it with a rag soaked with 5% carbolic acid, wipe the table or ground with a rag soaked with 5% carbolic acid, and then continue the operation after wiping your hands with an alcohol cotton ball.
6.工作结束，立即将台面收拾干净，将不应在无菌室存放的物品和废弃物全部拿出无菌室后，对无菌室用5%石炭酸喷雾，或开紫外线灯照射30min。
6. at the end of the work, clean up the table immediately, and remove all the articles and wastes that should not be stored in the asepsis room, then use the 5% carbolic acid spray or the ultraviolet lamp to irradiate 30min.
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